Date

2024-01-03

Protocol

Protocol changed

Item Changed

Secondary outcome

Previous Version

1. Nutritional status will be assessed using 1. Malnutrition Universal Screening Tool (MUST) 2. Body Mass Index (BMI) 3. Serum albumin level 4. Heamoglobin level. (Hb) (<35g/l and Hb - <10g/dl respectively will define poor nutritional status). 2. Psychological assessment will be done using 1. The Hospital Anxiety and Depression Scale (HADS) 2. Mindful Awareness Attention Score (MAAS) 3. Perceived Stress Scale 4. Quality of life (WHOQOL-BREF) questionnaire. 3. Biomarker assessment will be done by measuring 1. Salivary ?-amylase 2. Serum ?-endorphin 3. Serum Cortisol 4. Interleukin-6 (IL – 6) 5. Tumor necrosis factor-alpha (TNF – ?) 6. Brain-derived neurotrophic factor (BDNF) 7. Total Antioxidant Capacity (TAC) 8. Reactive Oxygen Species (ROS) level.

Next Version

1. Hand grip strength has recently been incorporated into the nutritional assessment protocol. Consequently, nutritional assessment will be conducted utilizing the Malnutrition Universal Screening Tool (MUST), Body Mass Index (BMI), Serum Albumin, Blood Hemoglobin (Hb) level, and the measurement of hand grip strength. 2. Biomarker assessment will only be conducted by measuring: 1. Serum ?-endorphin 2. Serum Cortisol.